Part:BBa_K1395002

sqr gene (sulfide quinone reductase) under constitutive promoter

The sqr gene encodes a protein of 427 amino acid residues with a theoretical molecular weight of 47 kDa. The sqr gene is expressed under a constitutive promoter and this enzyme converts the sulphide (S-2) to elemental Sulfur. Its expression is based on the availability of RNA polymerase holoenzyme and the expression is not affected by any transcription factors and is 3A assembly (RFC10) compatible. The Constitutive Promoter (BBA_J23119) is the "consensus" promoter sequence and the strongest member of the constitutive promoter family developed by John Christopher Anderson of UC Berkeley. This promoter can be used to tunes the expression level of constitutively expressed parts.

This part codes for the protein sulfide quinone reductase which is a FAD dependent oxidoreductase. Sulfide-quinone reductase (SQR),an ancient flavoprotein, is obligatory for growth on sulfide as hydrogen donor in photo and chemolithoautotrophic bacteria. It is a unique enzyme which is responsible for transfer of electrons from sulfide into the quinone pool. This enzyme converts the sulphide to Sulfur by the reaction.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 390